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Succinylation was assessed in IMAC phosphoproteome data using MSFragger-Labile in FragPipe, with PeptideProphet rescoring, PTMProphet localization (probability 0.75) and TMT-Integrator quantification

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The membrane was washed with PBST, and probed with HRP-conjugated anti-rabbit antibody (Jackson ImmunoResearch, West Grove, PA) in PBST containing 5% milk

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doi: 10.1016/j.xfre.2022.12.003 132 DabravolskiSANikiforovNGEidAHNedosugovaLVStarodubovaAVPopkovaTVet al

Your body adapts