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Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

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Compounded medications allow flexibility in dosing and delivery that supports individualized therapya particular advantage when starting low and titrating gradually to minimize side effects

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Symptoms of true copper toxicity include belly pain, nausea, chills, weakness, and a metallic taste in the mouth