Metabolome analysis by LC-MS/MS 3 10 6 million cells were washed in 1 ml of PBS, pelleted at 1500 rcf in an Eppendorf centrifuge at 4 o C, and resuspended in 100 l of 80% methanol (80 C) and 10 l of 0.3 mM 5-thio-glucose, used as internal standard to allow correction for sample recovery
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[DOI] [PMC free article] [PubMed] [Google Scholar] 192.Schremmer B., Manevich Y., Feinstein S.I., Fisher A.B