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The activation of luciferase reporters was measured by fold-change versus control

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For a 50mg vial of 5-Amino-1MQ with a target dose of 5mg per injection: 50mg 5mg = 10 doses 10 doses 0.25mL = 2.5mL bacteriostatic water This creates a 20mg/mL solution

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Like JNK, the activity of ASK1 is low in nonstressed cells due to its sequestration via protein: protein interactions with GSTM1 (GSTM1: ASK1 complex), and/or thioredoxin (Trx:ASK1 complex) (Cho et al ., 2001