Methylated & Non-methylated pUC19 DNA Set Cell & Soft Tissue DNA PCR fragment cloning

PCR fragment cloning

high level protein expression or over-expression is not always consistent and repeatable for every protein

This prepares the columns or plates for the efficient removal of PCR inhibitors such as polyphenolics

and Torulopsis

Competent Cells - DH5 Alpha are premade E

Methylated & Non-methylated pUC19 DNA Set Cell & Soft Tissue DNA PCR fragment cloningThe Methylated & Non methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a spike in control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non methylated DNA for assay calibration. The non methylated pUC19 DNA is pUC19 isolated from a methylation negative strain of bacteria (Dam, Dcm), and the methylated pUC19