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Two days after siRNA transfection, cells were transfected with an additional 0.5 l PDS5B siRNA (jkSIR012: 20 M), 1.2 g HA-TwinStrepTagII-PDS5B wild type or the 17 A mutant, 0.8 g I-SceI expression vector (pCBASce), and 5 l Lipofectamine 2000

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