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Acknowledgements This work was supported by the National Natural Science Foundation of China (82073313 to D.L.), the joint project of the State Administration of Traditional Chinese Medicine and Health Commission of Shandong Province (GZY-KJS-SD-2023-094), the Traditional Chinese Medicine Special Project of Binzhou Medical University Affiliated Traditional Chinese Medicine Hospital (2023ZYZX02) and the 2023 Qilu Biancang Traditional Chinese Medicine Talent Cultivation Project (to D.L.)

The suspended brain cubes were centrifuged at 100 g, 4 C for 20 sec to remove medium, and washed once with the medium

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