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The thermal cycler was programmed for 40 cycles of denaturation at 95 C for 30 s, annealing at 58 C, and extension at 72 C for 30s

While both copper peptides stimulate fibroblast activity and extracellular matrix remodeling , AHK-Cu shows enhanced effects on dermal papilla cells specifically

Additionally, immunohistochemical staining and enzyme-linked immunosorbent assays were performed to examine A-associated pathological changes

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