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A siliconTeflon monofilament 0.13 mm diameter with a 0.3 mm diameter heat-blunted end was used as the occluding suture

The Journal of Nutrition, 134(3), 489-492

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The gradient elution program was as follows: starting at 32% B, linear increase to 55% B from 0-5 min, followed by linear increase to 65% from 5-8 min, hold at 65% B from 8-10 min, linear increase to 80% B from 10-14 min, linear increase to 90% B from 14-25 min, linear increase to 100% B from 25-25.5 min, hold at 100% B until 27 min, drop to 32% B at 27.1 min and equilibration until the 30 min mark