To explore the interactions between AvrPto and neighboring immune system proteins, the authors designed four constructs: AvrPto_BirA (fusion of AvrPto with the N-terminus of BirA), MP_BirA (fusion of an 8-amino acid AvrPto myristoylation/palmitoylation (MP) motif with the N-terminus of BirA), BirA alone, and an empty vector (EV) control
It was given (i) intraperitoneally or (ii) intragastrically or (iii) topically at the swollen brain through an additional midline burr hole placed approximately 2 mm rostral to the horizontal line connecting the two parasagittal burr holes, making a triangle with them and overlying the sagittal sinus in the dural
It belongs to the same class of compounds as BPC-157, Sermorelin, and other therapeutic peptides, though it works through an entirely different receptor system
bioRxiv (2023) 08.17.553657
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Methods: A comprehensive search was conducted across PubMed, Scopus, and Embase up to July 2024