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The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm

doi: 10.1056/NEJMra1110560

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Chow-fed mice with KO of Gipr in GABAergic neurones achieved by crossing Gipr fx/fx with VGAT-Cre strains, were also previously reported to have normal body weight, although with subtle differences in fat mass and respiratory exchange ratio (RER), and improved glucose metabolism 5,11

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There are at least three inborn errors in the pentose phosphate pathway that have been identified