The affinity of the PV3 wild type was 51.0 nM, whereas the mutants K87pAcF, C23pAcF, S85pAcF and N77pAcF showed a significant increase in binding affinity
Mice were housed in the animal facilities at the University of Illinois at Chicago, Research Resources Center, and the studies were approved by the Institutional Animal Care and Use Committee, which is certified by the American Association of Accreditation of Laboratory Animal Care
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The cells were then treated six more times with 35 M