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The half-life of NO is extremely short, lasting only 2-4 seconds

At the cellular level, glutathione functions as a cofactor for antioxidant enzymes like glutathione peroxidase and glutathione-S-transferase, which neutralize reactive oxygen species and support phase-II detoxification pathways

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Then, plates were washed with PBS, fixed with 4% paraformaldehyde, permeabilized by 0.2% Triton X-PBS solution, repeatedly washed by PBS, and stained with FITC-labeled phalloidin to bounds with the F-actin ring in cells for 1 h in darkness

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