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Methods Cell viability, Apoptosis, activation and inflammatory responses of microglia, expressions and activity of Shh signaling pathway proteins were detected by Cell Counting Kit 8, Flow Cytometry, immunocytochemistry, ELISA, and Western blotting, respectively

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Batch Purity Report Janoshik Verified Current Batch: Pending first shipment Every batch of Epithalon sold by Pepspan is independently tested by Janoshik Analytical (Prague, Czech Republic)

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Also discard if it has been more than 28 days since reconstitution or if the vial has been stored at room temperature for extended periods