2.6 Protein sample preparation for mass spectrometry Protein samples (10 g/sample) were reduced with 5 mM Tris(2-carboxyethyl) phosphine and alkylated with 10 mM 2-chloroacetamide at room temperature for 20 min
The picture that emerges from these experiments is that endogenous GSH is not just an inhibitor of inflammation, but it is required to allow a proper response to infection, and direct the migration of inflammatory PMN away from the lung, where they cause ARDS, and towards the site of infection, where they kill bacteria (Figure 6)
Endogenous PTHrP plays an important role in fracture healing as demonstrated in an PTHrP haplo-insufficiency model where reduced cartilaginous and bony callus formation was noted together with reduced endochondral and osteoblastic bone formation [229]
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