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Insufficient hydration levels could impair metabolic processes and contribute to adverse reactions following the injection

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Vandenbroucke-Grauls, C

Mild fatigue during the first week as the body adjusts to altered metabolic signaling

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After an additional twenty-four hours, media was exchanged with fresh DMEM +dFBS / RPMI +pyruvate +dFBS /McCoys 5A +dFBS that was incubated overnight at 5% CO 2 supplemented with 300 ng/mL of Dox (for HeLa and Caki-1 lenti lines)