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Brain punches were obtained using a cryostat, and RNA was extracted using the Promega RNA extraction kit

However, significant translational barriers remain: no human pharmacokinetic data, high synthesis cost, unknown long-term safety, and the absence of clinical trials

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Cell lines were regularly tested for mycoplasma contamination using a MycoStrip Mycoplasma Detection Kit (InvivoGen rep-mys-10)

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