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After treatment, to assess mitochondrial membrane potential ( m ), A375 cells were incubated with 4 M JC-1 (ThermoFisher Scientific) in phenol red free DMEM for 20 min in a 5% CO 2 incubator at 37 C, then washed twice with DMEM without phenol red
Additionally, during liver processing, glutathione helps counteract secondary free radicals, further reducing oxidative stress
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This is a significant advantage over many injectable peptides that require subcutaneous administration
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