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(PubMed) Steinbrecher A, Meplan C, Hesketh J, et al

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Agrobacterium cells were collected by centrifugation, resuspended in infiltration buffer (10 mM MgCl, 200 M acetosyringone, 10 mM MES) to an OD of 0.70.8, and incubated at room temperature for 2 h