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It is typically an intramuscular injection in the arm, and is given by a nurse or a doctor

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Melatonin can pass through the placental barrier and enter the fetal circulation to promote fetal growth and entrain the circadian rhythm of the fetus (34, 69)

Biomarkers tested Beyond behavioral testing, the researchers conducted extensive analysis of brain tissue, measuring various crucial markers: [1] Brain-derived neurotrophic factor (BDNF) Key neurotransmitters (acetylcholine, dopamine, GABA) Antioxidant markers (catalase, glutathione) Beta-amyloid levels Cyclic GMP This comprehensive approach allowed the research team to not only observe behavioral outcomes but also understand the underlying neurochemical changes driving these effects