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Nutrients 8 , 56 (2016)

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Evaluation of AsA/DHA and GSH/GSSG The total AsA, reduced AsA, and oxidized DHA levels were measured according to the method of 2 HPO 4 buffer (containing 5 mM of EDTA, pH 7.4) in the presence of either 50 l of 10 mM of dithiothreitol (for the total AsA) or 50 l of water (for the reduced AsA)

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The temporary nature of results combined with the need for continuous treatment represents a significant consideration for those evaluating glutathione IV therapy for skin brightening purposes