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In contrast to -glutamylcysteine synthetase, glutathione synthetase accepts a large variety of glutamyl-modified analogs of -glutamylcysteine, but is much more specific for cysteine-modified analogs of -glutamylcysteine

Understanding why this variability exists requires examining the mechanisms more closely

Cloudiness, a yellow or brown tint, or floating particles usually means it has degraded or been contaminated

2b Using a two-chamber gradient maker Using a two-chamber gradient maker The traditional way of constructing a continuous gradient is to use a standard two-chamber gradient maker (Figure 3)

Compounds thereof AHUMAN NECESSITIES A61MEDICAL OR VETERINARY SCIENCE

doi: 10.1016/0162-3109(89)90020-9