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Plasmids, subcloning and creation of NNK libraries Genes, codon optimized for expression in S

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Immunostaining revealed mixed cell populations with many undifferentiated hPSCs and mesoderm cells, and DE markers SOX17 and FOXA2 were expressed at lower levels compared to the Activin A-based control (CA)

R min and R max were obtained by fitting the R values and the lysate-based GSH concentrations C GSH using equation: ( R R min )/( R max R )= k C GSH , in which k is a constant

Its amino acid seque nce is Gly-Glu-Pro-Pro-Pro- Gly-Lys-Pro-Ala-Asp-Asp-Ala- Gly-Leu-Val