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Reaction mixtures containing total RNA (500 ng of each), 0.2 mM dNTPs, 0.2 M of each primer and an enzyme mixture composed of SuperScript II RT, Platinum Taq DNA polymerase and 1X buffer with 1.2 mM MgSO4 were maintained at 50C for 20 min, then at 94C for 2 min and PCR was performed as follows: 30 cycles at 94C for 15 sec, 55C for 30 sec and 70C for 30 sec

They happened because participants followed a precise, structured dosing schedule that gradually increased their injection amounts over months, giving the body time to adapt while maximizing the therapeutic effect

Senyawa ini memindahkan asam lemak rantai panjang dari sitoplasma ke mitokondria

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