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To ensure optimal staining conditions, the slides were subjected to three consecutive washes with phosphate-buffered saline (PBS), each lasting five minutes

However, a new case report highlights a hidden side effect associated with this popular medication, revealing how the drug appears to sap patients motivation and energy, resulting in emotional flatness." In addition to altering users metabolism, GLP-1s treat obesity by reducing the incentive to eat through the brain's dopaminergic reward system

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Sexual function changes that are primarily neurochemical, meaning dopamine or serotonin mediated, typically resolve within one to two months of discontinuation as the brain reward system recalibrates

Acknowledgments During the preparation of this manuscript, the authors used OpenAI (GPT-5.2.) for scientific language refinement and grammatical correction

J Clin Endocr Metab 85:671-675, 2000