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Bacterial secretion of histamine within the gut influences immune responses within the lung

They were permeabilized with 0.1% Triton X-100 for 15 min and then incubated with 25 ng/mL RNase for 1 h at 37 C to remove unwanted RNA contamination and enhance the specificity of the staining

The effect of buffer pH on the rate and efficiency of Zn(II) release during the reaction of MT2 with allicin was also investigated (Fig