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Cell viability Cell viability was determined by the CCK8 method according to the manufacturers protocol

Western blot analyses further demonstrated that Anaerococcus transplantation enhanced IGF-1/PI3K/mTOR signaling and reduced apoptosis, whereas rapamycin markedly weakened MOS-mediated pathway activation, thereby partially abrogating its protective effects ( Figure 5E )

Watt FE

The study demonstrated that tazarotene treatment led to a significant reduction in hyperpigmentation rates

Apoptosis assay Apoptosis was detected using the Annexin V-FITC Apoptosis Detection Kit (Beyotime) according to the manufacturers instructions

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