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For quantitative real-time polymerase chain reaction (qPCR), samples were assayed in duplicate using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Hercules, CA)

Because the peptide has a short half-life in circulation estimated around 1 to 2 hours based on exercise-induced measurements consistent dosing is important for maintaining its metabolic effects

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When prescribed by qualified clinicians with appropriate baseline testing and monitoring, combination therapy is generally well-tolerated