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After two PBS washes, the cells were treated with RNase A (Gibco, Grand Island, NY) for 30 min at 4 C to remove RNA and ensure only DNA is stained with propidium iodide (PI) (Sigma-Aldrich)

Store in a dry, cool, and shaded place

These junctions are composed of proteins, including occludin, claudin and junctional adhesion molecules (JAMs)

In addition, due to such compensation, First Trust has an incentive to sell private investments issued by FTCM or offered through the First Trust Private Investment Platform, resulting in a material conflict of interest which should be considered when making a decision to invest in private funds offered by or through FTCM

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