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Following serum starvation, cocktails comprised of vehicle and HGF (2.5 ng/ml) with/without the test compound were prepared and pre-incubated for 30 minutes at room temperature

Different patterns of plasma medium and long chain acylcarnitine alterations usually occurred with other biomarkers of mitochondrial energy metabolism (Figure 5, groups 2, 5, and 7)

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However, in conditions like ischemic stroke and spinal cord injury, CHAC1 promotes neuronal damage by inducing ferroptosis through the depletion of GSH, a critical antioxidant, exacerbating oxidative stress and worsening the injury (Mei et al., 2017