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Briefly, 1 mg of protein was added on the IPG strip (pH 47, 24 cm), and the pre-programmed power supply (EPS 3500l, Amersham Pharmacia Biotech) was started immediately

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Figure 7 Functionally, MAPKAPK2 depletion significantly exacerbated H 2 O 2 -induced oxidative stress, as evidenced by increased intracellular ROS accumulation, with ROS levels increasing by approximately 23 fold compared with the control group