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The pellet was dissolved in 100 l RIPA buffer and protein content was determined

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From these molar concentrations the status of the GSSG/2GSH couple was calculated using the Nernst equation E h c = 255 30 log ([GSH] 2 / [GSSG]) in mV [26], assuming an intracellular pH of 7.25 for RBCs and a temperature in vivo of 37 C [27]

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If you want to understand how those broader markers fit together, our guide to how gut health affects thyroid function is a useful next read

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