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Waring, J

For gene expression analysis, 1 g of RNA was used for cDNA synthesis using qScript cDNA Synthesis Kit (Quanta Bio, #66196756)

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[58] FDA further explains that the submission of an MDR itself does not necessarily demonstrate that the device caused or contributed to the adverse outcome or event

Immediately after separation, each mouse received the dutasteride-treated diet (~ 13 g/g BW, a dose shown previously to affect 5 reductase activity in the brain [104]) or a similar amount of vehicle-treated diet for controls