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doi: 10.1155/2021/9991001 99 HuangLHongYFuXTanHChenYWangYet al

Western blotting Proteins from tissues or cells were prepared in RIPA buffer (50 mM pH 7.4 Tris-HCl, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 1% Nonidet P (NP)-40 (NP-40), 1 mM phenylmethanesulfonyl fluoride (PMSF), 1 mM dithiothreitol (DTT), and 1X protein inhibitor cocktail (PIC))

The transport of L-lactate is mainly mediated by MCT1 and MCT2, which facilitate its influx from the extracellular space, while MCT4 promotes its efflux, regulated by the lactate concentration gradient [40-42]

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Acts as a signaling molecule that inhibits transforming growth factor beta-1 (TGF-1), a cytokine that triggers premature catagen phase entry

Purified IDE was incubated without and with 10 4 M GSNO and GSNO(ox) for 2 hours at 37C in 100 mM Tris, pH 7.5