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PMID 17766407

Sometimes this issue is fixed simply by optimizing buffers, for instance by increasing salt or detergent concentration, so that the interacting protein no longer binds to the GST only control, but still binds to your GST-fusion protein of interest

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The timeframe for observing results also plays a role short-term effects might call for a single, higher dose, while long-term studies may benefit from smaller, repeated doses

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