The enrichment analysis of the significantly more abundant KOs in T2, T3 and T4 indicated that carbon ( metF , kdgK and abfD ), pyrimidine ( pydC and ushA ) and cysteine/methionine ( AHCY , mtnA ) were enriched in T2, arginine, alanine, aspartate and glutamate metabolism ( argB , argG , glnA ) and the pentose phosphate pathway ( TALDO1 and PRPS ) were enriched in T3 and thermogenesis/oxidative phosphorylation ( CYTB , ATPeF0A , ND1 , ND2 and ND6 ), the two-component system/ lysine degradation/ aminobenzoate degradation/ butanoate metabolism ( atoD , atoA , CYTB , tctA and kdd ) and the metabolism of glycine, serine and threonine ( kbl and thrH ) and the degradation of flavonoids ( phy ) were enriched in T5
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For all practical lab calculations, you can assume the final volume of the solution is equal to the volume of the bacteriostatic water you added
