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HRP-labeled rabbit anti-rat IgG (ab6734) was purchased from Abcam (Cambridge, MA, USA)
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The analyte and internal standard were extracted from 50 l of plasma by solid phase extraction
Their website offers a wealth of knowledge and advice on the science and terminology of research peptides
To assess metallothionein protein abundance in response to SLC30A1 depletion, we selected metallothionein 1E ( MT1E ) as a reporter gene and inserted an artificial exon encoding for mScarlet within the first intron of the MT1E gene in the 1321N1 cell line (Fig S4B)
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