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These seniors are facing poverty and social isolation that puts them at "We Are Family coordinates monthly grocery deliveries to 550 seniors in the Columbia Heights/Park View and North Capitol Street/Shaw neighborhoods

doi: 10.1007/978-3-030-80682-8_6 36 EleftherianosIHeryantoCBassalTZhangWTettamantiGMohamedA

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The GST-tagged Sh HTL7 was purified similarly using 50 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM DTT, with 0.1% (v/v) Tween-20 added for lysis, and loaded on Glutathione Sepharose 4B (Cytiva) resin