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The slides were mounted with Fluoprep (BioMerieux), and immunofluorescence was viewed and analyzed using an Imager Z1 fluorescence microscope equipped with a Digital Camera (Zeiss, Jena, Germany) connected to an AxioVs40 software (Zeiss), and with an LMS800 confocal microscope piloted by a ZEN 2.3 software (Zeiss)

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Besides this newly identified L1FnIII-2 intra-protomer interaction, there is an L1FnIII-2 inter-protomer interaction that already exists in the apo state of IGF1R and persists in the active state

The activation of GLP-1R by a specific agonist induces an increase in intracellular cAMP levels, which leads to channel opening

It also binds to the copper in tyrosinase, blocking its activity and eumelanogenesis

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