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The intramuscular route ensures consistent, reliable delivery of B12, independent of gastrointestinal absorption

The Boc-protected cystine-1,1- 13 C was reacted with two equivalents of glycine tert -butyl ester (OtBu) hydrochloride using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide monohydrochloride and 1-hydroxybenzotriazole to produce the N and C-protected dipeptidyl (Boc-Cys-Gly-OtBu) disulfide derivative

Extrapolation of these doses to humans, using standard allometric conversion factors (factor of 6.2 for rats), yields theoretical doses on the order of 1.6 g/kg in humans

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