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After three washes with PBS, wells were incubated with 1:500 dilution of sheep polyclonal anti-human C4bp (PC026, The Binding Site) in 1% fatty acid free BSA in PBS for 1h at RT, then washed thrice with PBS

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Method selection should be driven by the required conjugation site, substrate electrophilicity, oxidation sensitivity, and whether the customer needs a metabolite-like adduct, a preserved free-thiol derivative, or a labeled construct for downstream assay use