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R., Balesh, A

The interface with the second largest buried surface area in the Tyk2 crystal lattice did place the C- and N-termini in close enough proximity to be linked by the interdomain linker, and this interface was investigated in detail and mutational data did support the biological significance of this interaction surface

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The His-tagged protein can then be eluted by adding imidazole, which competes with the histidine residues for binding to the nickel

doi: 10.1530/eje-19-0171 50 NauckMAHeimesaatMMBehleKHolstJJNauckMSRitzelRet al