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However, under pathological states such as obesity, chronic inflammation, and mitochondrial dysfunction, impaired BCAA catabolism leads to accumulation, participating in the pathogenesis and progression of insulin resistance, type 2 diabetes, cardiovascular disease, and sarcopenia through multiple mechanisms including excessive mTORC1 activation, insulin signaling interference, and mitochondrial stress induction

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Stain the sections with the Fast Green solution for 5 min

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