Park, S.-Y
For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min
Enables isotope pattern analysis for precision confirmation
subtilis is a fused bifunctional enzyme with GTP cyclohydrolase II and 3,4-DHBP synthase activities, which catalyzes the cleavage of GTP and converts DHBP from Ribu5P in the initial steps of both branches of riboflavin biosynthesis (Figure 1)
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You can expect the session to take about thirty to forty five minutes