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Description

Zinquin-stained cells were washed with FACS buffer and observed immediately by microscopy with excitation at 361367 nm and emmission at 482488 nm

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Before injection, the larvae were anesthetized in 0.05% tricaine solution for 2 min and then positioned ventral side up in grooved agarose molds

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(Shiga, Japan), used for colony PCR amplifications

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Kornhuber J, Mack Burkhardt F, Riederer P, Hebenstreit GF, Reynolds GB, et al

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