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The pH of the mobile phase media can be adjusted to neutral or basic values using ammonium buffers on the same carbon media

Isgren A, Sellgren C, Ekman CJ, Holmn-Larsson J, Blennow K, Zetterberg H, et al

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(107) demonstrated that even though prednisolone increased serum total and bioactive IGF-I, post-IGF-IR signaling was inhibited indicating that prednisolone-induced increases in serum IGF-I levels is not accompanied by increased IGF-I action