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Further validation of the molecular mechanisms was performed using flow cytometry, Western blotting, and reverse transcription quantitative polymerase chain reaction (RT-qPCR)
Compression- or actomyosin-mediated nuclear flattening alters the permeability across nuclear pore complexes, resulting in active nuclear import of key transcription regulators such as YAP, SMAD3, Twist1, and Snail [124, 125]
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