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doi: 10.1007/s12185-017-2366-2 61 Garca-CobosS.MoscosoM.PumarolaF.ArroyoM.LaraN.Prez-VzquezM.et al

May 1990

HC neutrophils were cultured in the presence of HC or SLE serum with or without the addition of Cl-amidine (Cl) (peptidyl arginine deiminase 4 (PAD4) inhibitor, 100 M), or LPX-1 (1 M) for 4 or 16 hours and NETs were assessed in SYTOX Green+ cells based on morphology (n = 6)

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Always use sterile Bacteriostatic Water or sterile lab-grade water for the reconstitution process