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The nucleophilic attack of the hydroxyl group of Thr-381 against the 5-carbonyl carbon of the -glutamyl moiety is facilitated by the -amino group of the same residue, which acts as a general acidbase catalyst generally found in the Ntn-hydrolase superfamily (Figure 5A)

However, changes in cellular health can interfere with this ratio

doi: 10.3109/03602532.2011.552913

Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 405 nm Precision pipettes to deliver 2 L to 1 mL volumes Multi-channel pipettes to deliver 20 L to 200 L volumes Tubes to prepare sample dilutions Ultrapure water 50 mL conical tubes Protocatechuic acid (HClO 4 ) (PCA) Potassium hydroxide (KOH) Assay Procedure Summary Add 50 L of the diluted GSSG Standards or unknown samples to the 96-well microtiter plate

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