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Our preliminary interpretation is that replacement of Tyr110 indeed affects the interaction with GSH but, as compared to K105A/Y, does not drastically alter the ratio between the steady-state concentrations of the deprotonated and glutathionylated enzyme species E and F, respectively

Wang C, Zhang J, Wu H, Li ZM, Ye Q (2015) Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production